基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因

doi:10.11843/j.issn.0366-6964.2016.01.004

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (1): 25-33.doi: 10.11843/j.issn.0366-6964.2016.01.004

基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因

束婧婷^#，章明^#，宋迟，单艳菊，徐文娟，宋卫涛，李慧芳^*

（江苏省家禽科学研究所，扬州 225125）

收稿日期:2015-01-22 出版日期:2016-01-23 发布日期:2016-01-23
通讯作者: 李慧芳，研究员，E-mail：lhfxf_002@aliyun.com
作者简介:束婧婷(1980-)，女，江苏宿迁人，博士，副研究员，主要从事家禽遗传育种研究，Tel：0514-85599018，E-mail：shujingting@163.com；章明（1978-），男，江苏姜堰人，硕士，副研究员，主要从事家禽遗传育种研究，E-mail：6259513@qq.com。二人共为第一作者
基金资助:
国家自然科学基金（31301967）；现代农业产业技术体系建设专项资金（CARS-42-G03）；江苏省自然科学基金（BK2012268）；江苏省农业科技自主创新资金项目（cx（15）1009）

Identification of Differentially Expressed Genes for Myofiber Characteristics in Soleus Muscles between Qingyuan Partridge Chickens and Cobb Broilers

SHU Jing-ting^#，ZHANG Ming^#，SONG Chi，SHAN Yan-ju，XU Wen-juan，SONG Wei-tao，LI Hui-fang^*

(Jiangsu Institute of Poultry Science，Yangzhou 225125，China)

Received:2015-01-22 Online:2016-01-23 Published:2016-01-23

摘要/Abstract

摘要：

对生长速度长期的高压选择导致了快大型肉鸡与优质型地方鸡显著的表型差异，本研究旨在研究这种表型差异的分子遗传机理。采用Agilent鸡全基因组表达谱芯片对达到性成熟的优质型清远麻鸡（112 d）和快大型科宝肉鸡(42 d)比目鱼肌中与肌纤维发育和类型组成相关的候选基因及信号通路进行系统筛查。结果，芯片分析共筛选到差异倍数在2倍及以上的基因1 318个，以科宝肉鸡作为参照，清远麻鸡中上调基因501个，下调基因817个，主要涉及到肌肉发育、能量代谢、脂质代谢等生物学过程。基于KEGG Pathway分析发现，差异基因除了参与肌纤维发育和分化相关信号通路（如Hedgehog信号通路和Ca²⁺信号通路）外，Wnt信号通路，mTOR信号通路，MAPK信号通路，ErbB信号通路、JAK-STAT信号通路等一些跟能量代谢相关的信号通路也被富集为显著的信号通路。与能量代谢相关的信号通路和与肌肉发育相关的信号通路相互作用形成一个调控网络从而影响肌纤维的发育，筛选出了20个在肌纤维生长发育及分化过程中可能具有重要影响的候选基因，但这些差异表达基因在肌纤维的发育和分化过程中的作用尚待深入研究。

Abstract:

In the modern chicken industry，fast-growing broilers have undergone strong artificial selection for muscle growth，which has led to remarkable phenotypic variations compared with slow-growing chickens.The present study was designed to explore the molecular mechanism underlying these phenotypes differences.Agilent cDNA microarray analyses were conducted to determine gene expression profiles of soleus muscle sampled at sexual maturity age of Qingyuan Partridge chickens (QY，112 d，a slow-growing Chinese breed with high meat quality) and Cobb broilers (CB，42 d，a commercial fast-growing broiler line).A systematic identification of candidate genes and new pathways related to myofiber development and composition in soleus muscles between the 2 breeds were analyzed.1 318 genes with at least 2-fold differences were identified in soleus muscles of QY and CB chickens.When slow-growing QY chickens were compared with fast-growing CB chickens，the expression of 501 genes were up-regulated，and 817 genes were down-regulated.These genes mainly were related to muscle development，energy metabolism or lipid metabolism processes.In addition，based on KEGG pathway analysis，it was found that in addition to pathways affecting myofiber development and differentiation (pathways for Hedgehog & Calcium signaling)，energy metabolism signaling pathways (Wnt signaling pathway，mTOR signaling pathway，MAPK signaling pathway，ErbB signaling pathway and JAK-STAT signaling pathway) were also enriched.Our data indicates that energy and muscle metabolism pathways might form a network，which influence the development of myofibers，20 genes were potentially related to myofiber development and composition.However，large scale analyses are still required to validate the role of the genes identified and ultimately to find molecular markers that can be used for selection or to optimize rearing practices.

中图分类号:

S813.2

束婧婷，章明，宋迟，单艳菊，徐文娟，宋卫涛，李慧芳. 基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因[J]. 畜牧兽医学报, 2016, 47(1): 25-33.

SHU Jing-ting，ZHANG Ming，SONG Chi，SHAN Yan-ju，XU Wen-juan，SONG Wei-tao，LI Hui-fang. Identification of Differentially Expressed Genes for Myofiber Characteristics in Soleus Muscles between Qingyuan Partridge Chickens and Cobb Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 25-33.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2016.01.004

https://www.xmsyxb.com/CN/Y2016/V47/I1/25

参考文献

［1］SHU J T，XU W J，ZHANG M，et al.Transcriptional co-activator PGC-1α gene is associated with chicken skeletal muscle fiber types［J］.Genet Mol Res，2014，13(1)：895-905.
［2］ASHMORE C R，DOERR L.Comparative aspects of musle fiber types in different species［J］.Exp Neurol，1971，31(3)：408-418.
［3］KARLSSON A H，KLONT R E，FERNANDEZ X.Skeletal muscle fibres as factors for pork quality［J］.Livest Prod Sci，1999，60：255-269.
［4］袁媛，刘月光，史新娥，等.调控骨骼肌纤维类型转化的信号通路［J］.中国生物化学与分子生物学报，2010，26(9)：796-801.
YUAN Y，LIU Y G，SHI X E，et al.Signaling pathways in skeletal muscle fiber-type transition［J］.Chinese Journal of Biochemistry and Molecular Biology，2010，26(9)：796-801.(in Chinese)
［5］PANNIER L，MULLEN A M，HAMILL R M，et al.Association analysis of single nucleotide polymorphisms in DGAT1，TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle［J］.Meat Sci，2010，85(3)：515-518.
［6］RAJ S，SKIBA G，WEREMKO D，et al.The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights［J］.Meat Sci，2010，86(2)：324-330.
［7］WEI L，LIJUAN H，DAN L.Microarray analysis of differently expressed microRNA profiles induced by UVB irradiated in mice skin［J］.J Invest Dermatol，2010，130：S132.
［8］WU T，ZHANG Z H，YUAN Z Q，et al.Distinctive genes determine different intramuscular fat and muscle fiber ratios of the longissimus dorsi muscles in Jinhua and Landrace pigs［J］.PLoS One，2013，8(1)：e53181.
［9］LI Y，XU Z，LI H，et al.Differential transcriptional analysis between red and white skeletal muscle of Chinese Meishan pigs［J］. Int J Biol Sci，2010，6(4)：350-360.
［10］ZHENG Q，ZHANG Y，CHEN Y，et al.Systematic identification of genes involved in divergent skeletal muscle growth rates of broiler and layer chickens［J］.BMC Genomics，2009，10：87.
［11］GUTH L，SAMAHA F J.Procedure for the histochemical demonstration of actomyosin ATPase［J］.Exp Neurol，1970，28(2)：365-367.
［12］DRANSFIELD E，SOSNICKI A A.Relationship between muscle growth and poultry meat quality［J］.Poult Sci，1999，78(5)：743-746.
［13］REHFELDT C，KUHN G.Consequences of birth weight for postnatal growth performance and carcass quality in pigs as related to myogenesis［J］.J Anim Sci，2006，84：113-123.
［14］RENAND G，PICARD B，TOURAILLE C，et al.Relationships between muscle characteristics and meat quality traits of young Charolais bulls［J］.Meat Sci，2001，59(1)：49-60.
［15］VIDAL-PUIG A，SOLANES G，GRUJIC D，et al.UCP3：an uncoupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue［J］.Biochem Biophy Res Commun，1997，235(1)：79-82.
［16］LOUIS M，VAN BENEDEN R，DEHOUX M，et al.Creatine increases IGF-I and myogenic regulatory factor mRNA in C₂C₁₂ cells［J］.FEBS Lett，2004，557(1-3)：243-247.
［17］CAGNAZZO M，TE PAS M F，PRIEM J，et al.Comparison of prenatal muscle tissue expression profiles of two pig breeds differing in muscle characteristics［J］.J Anim Sci，2006，84(1)：1-10.
［18］ANAKWE K，ROBSON L，HADLEY J，et al.Wnt signalling regulates myogenic differentiation in the developing avian wing［J］.Development，2003，130(15)：3503-3514.
［19］EVANS R M，BARISH G D，WANG Y X.PPARs and the complex journey to obesity［J］.Nat Med，2004，10(4)：355-361.
［20］ARANY Z，LEBRASSEUR N，MORRIS C，et al.The transcriptional coactivator PGC-1beta drives the formation of oxidative type IIX fibers in skeletal muscle［J］.Cell Metab，2007，5(1)：35-46.
［21］MAHLAPUU M，JOHANSSON C，LINDGREN K，et al.Expression profiling of the γ-subunit isoforms of AMP-activated protein kinase suggests a major role forγ3 in white skeletal muscle［J］.Am J Physiol Endocrinol Metab，2004，286(2)：E194-E200.
［22］MCBRIDE A，GHILAGABER S，NIKOLAEV A，et al.The glycogen-binding domain on the AMPK beta subunit allows the kinase to act as a glycogen sensor［J］.Cell Metab，2009，9(1)：23-34.

[1]	王元清, 王兢, 朱波, 陈燕, 徐凌洋, 王泽昭, 张路培, 高会江, 李俊雅, 高雪. 基因组选配研究及其在畜禽育种中的应用[J]. 畜牧兽医学报, 2024, 55(1): 1-10.
[2]	李桃桃, 金美林, 费晓娟, 王慧华, 陆健, 狄冉, 魏彩虹. Hox基因家族及其对动物脊椎形成的影响[J]. 畜牧兽医学报, 2022, 53(4): 999-1009.
[3]	刘悦, 薛翔澜, 李晓波, 蒋琳, 浦亚斌, 何晓红, 马月辉, 赵倩君. 染色质开放性与动物胚胎发育关系的研究进展[J]. 畜牧兽医学报, 2022, 53(3): 680-687.
[4]	袁泽湖, 葛玲, 李发弟, 乐祥鹏, 孙伟. 整合生物学先验信息的全基因组选择方法及其在家畜育种中的应用进展[J]. 畜牧兽医学报, 2021, 52(12): 3323-3334.
[5]	谢宁, 张凯艺, 阮进学, 陶聪, 吴添文, 王彦芳, 杨述林. Glut4突变调控脂肪重分布及肌纤维重塑的机制研究[J]. 畜牧兽医学报, 2021, 52(9): 2464-2474.
[6]	王卫振, 邓占钊, 辛国省, 蔡正云, 顾亚玲, 张娟. 环状RNA的生物学功能及其在家禽中的研究进展[J]. 畜牧兽医学报, 2021, 52(7): 1778-1788.
[7]	牛化欣, 常杰, 胡宗福, Wang Yuxi. 基于组学技术研究反刍动物瘤胃微生物及其代谢功能的进展[J]. 畜牧兽医学报, 2019, 50(6): 1113-1122.
[8]	盛中伟, 单艳菊, 束婧婷, 章明, 姬改革, 屠云洁, 范建华, 邹剑敏. CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析[J]. 畜牧兽医学报, 2017, 48(10): 1825-1832.
[9]	营凡, 李鹏, 邢思远, 李庆贺, 郑麦青, 刘冉冉, 崔焕先, 文杰, 马友记, 赵桂苹. 不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析[J]. 畜牧兽医学报, 2017, 48(8): 1529-1534.
[10]	王杰, 刘璐, 刘杰, 赵桂苹, 刘冉冉, 郑麦青, 文杰. 基于通路分析方法诠释肉鸡体重性状全基因组关联研究[J]. 畜牧兽医学报, 2017, 48(5): 810-817.
[11]	金连丰，于广璞，孙霞，李庆章，高学军，崔英俊. 牛GHR基因与miR-139靶向关系的验证[J]. 畜牧兽医学报, 2016, 47(12): 2398-2404.
[12]	章杰，罗宗刚. 纯种与杂种猪肌肉生长相关分子表达的差异分析[J]. 畜牧兽医学报, 2016, 47(12): 2370-2378.
[13]	栾兆进，刘开东，贺建宁，程明，曲绪仙，柳楠. 绵羊FAM134B、PPARγ、HSL和FAS基因表达量及与肌内脂肪含量的关系[J]. 畜牧兽医学报, 2016, 47(12): 2379-2389.
[14]	亓利思，佟慧丽，李树峰,胡倩，严云勤. 牛结蛋白基因启动子的初步改造研究[J]. 畜牧兽医学报, 2016, 47(12): 2405-2413.
[15]	张进威，龙科任，王讯，李明洲，马继登. 环状RNA研究进展[J]. 畜牧兽医学报, 2016, 47(11): 2151-2158.

基于表达谱芯片挖掘清远麻鸡和科宝肉鸡比目鱼肌差异表达基因

Identification of Differentially Expressed Genes for Myofiber Characteristics in Soleus Muscles between Qingyuan Partridge Chickens and Cobb Broilers

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价